Buy Orexin-B Europe
Buy Orexin-B (Hypocretin-2) Europe. 28-amino acid wakefulness neuropeptide for sleep and OX2R research. ≥99% purity. Fast EU delivery.
What is Orexin-B?
Orexin-B (Hypocretin-2) is a 28-amino acid linear neuropeptide produced by lateral hypothalamic neurons alongside Orexin-A. Both peptides are derived from a common precursor, prepro-orexin, but Orexin-B lacks the disulfide bonds and pyroglutamyl cap of Orexin-A, giving it a distinct receptor selectivity profile. For published research, see PubMed studies on Orexin-B{:target=“_blank” rel=“noopener”}.
Orexin-B shows approximately 10-fold preferential binding to the OX2 receptor, which is strongly expressed in wake-promoting centres such as the tuberomammillary nucleus. This receptor selectivity makes Orexin-B a valuable probe for dissecting the contributions of OX2R versus OX1R to wakefulness and other orexin-regulated functions.
Best-Peptides supplies research-grade Orexin-B with guaranteed ≥99% purity and full analytical documentation.
How Does Orexin-B Work?
OX2R-Preferential Signalling
- OX2R selectivity - ~10-fold preference over OX1R
- Gq protein coupling - Phospholipase C activation at OX2R
- Calcium signalling - IP₃-mediated intracellular Ca²⁺ release
- PKC activation - Downstream protein kinase C cascades
Wake-Promoting Pathways
- Tuberomammillary nucleus - Histaminergic neuron activation via OX2R
- Locus coeruleus - Noradrenergic arousal modulation
- Cortical activation - Enhanced cholinergic tone
- Sleep-wake transitions - State boundary stabilisation
Neuroendocrine Integration
- Energy homeostasis - Metabolic rate regulation
- Autonomic control - Sympathetic output modulation
- Stress response - HPA axis interaction
Research Applications
Sleep and Arousal
- OX2R pharmacology - Receptor-selective functional studies
- Narcolepsy research - OX2R-specific aspects of orexin deficiency
- Sleep architecture - NREM/REM transition modulation
Receptor Selectivity Studies
- OX1R vs OX2R - Comparative agonist profiling
- Orexin-A comparison - Functional selectivity research
- DORA pharmacology - Dual orexin receptor antagonist studies
Neuroendocrine and Metabolic Research
- Feeding regulation - Appetite and satiety pathway studies
- Thermoregulation - Core body temperature modulation
- Autonomic nervous system - Peripheral metabolic effects
Orexin-B Specifications
| Specification | Detail |
|---|---|
| CAS Number | 205640-91-1 |
| Molecular Formula | C₁₂₅H₂₀₄N₄₀O₃₇ |
| Molecular Weight | 2937.3 g/mol |
| Sequence | RSGPPGLQGRLQRLLQASGNHAAGILTM-NH₂ |
| Purity | ≥99% (HPLC) |
| Appearance | White lyophilised powder |
Reconstitution and Handling
Reconstitution Protocol
- Allow vial to reach room temperature
- Add bacteriostatic water slowly down vial wall
- Gently swirl—do not shake
- Solution should be clear
Storage Recommendations
| Condition | Temperature | Duration |
|---|---|---|
| Lyophilised | -20°C | Up to 24 months |
| Reconstituted | 2-8°C | Up to 4 weeks |
Ordering Information
| Size | Price |
|---|---|
| 10 × 5 mg | €450 |
Minimum order: €200 | 10% discount on orders over €200
Why Choose Best-Peptides for Orexin-B?
- Guaranteed ≥99% purity - HPLC verified
- Full documentation - COA with every order
- EU laboratory tested - Quality assured
- Fast delivery - Express shipping available across Europe
- Research support - Technical assistance
Research Use Statement
For laboratory research only. Not intended for human or veterinary use. Researchers should follow all applicable regulations.
Specifications
| CAS No. | 205640-91-1 |
|---|---|
| Molecular Weight | 2937.3 g/mol |
| Purity | ≥ 99% |
| Storage | -20°C recommended (research-only) |
| Available Sizes | 10 × 5 mg vials |
| Price Range | €450 per pack |
FAQ
Orexin-B (Hypocretin-2) is studied for its role in wakefulness regulation, sleep architecture, energy homeostasis, and neuroendocrine control. Research focuses on OX2R-selective signalling, narcolepsy pathophysiology, and arousal state transitions.
Orexin-B is a 28-amino acid linear peptide without disulfide bonds, making it structurally simpler than Orexin-A. It shows approximately 10-fold selectivity for OX2R over OX1R, whereas Orexin-A binds both receptors with similar affinity.
Orexin-B preferentially activates OX2R, a Gq-coupled GPCR that stimulates phospholipase C, increasing intracellular calcium and activating PKC. OX2R is strongly expressed in the tuberomammillary nucleus, a major histaminergic wake-promoting centre.
Store lyophilised Orexin-B at -20°C for long-term stability. Once reconstituted, keep at 2-8°C and use within 4 weeks. Protect from light and avoid repeated freeze-thaw cycles.
Our Orexin-B is ≥99% pure as verified by HPLC. Each batch includes a Certificate of Analysis with purity data and mass spectrometry identity confirmation.
Yes, comparative studies of Orexin-A and Orexin-B are common in receptor selectivity and functional assay research. Both peptides are available from Best-Peptides for comprehensive orexin system characterisation.
Reconstitute in sterile bacteriostatic water or PBS. Add solvent slowly along the vial wall and gently swirl to dissolve. Orexin-B dissolves readily in aqueous solution.
Orexin-B is available in 5 mg vials, supplied as a pack of 10 × 5 mg for research applications.
Customer Reviews (12)
Based on 12 reviews
Dr. Andreas Keller • 2026-03-13Verified Purchase
Orexin-B reliably activates OX2R in our calcium mobilisation assays. Selectivity over OX1R is clearly demonstrated. Top-quality product.
Utrecht Sleep Science Lab • 2026-02-27Verified Purchase
Orexin-B's preferential OX2R binding is confirmed by our radioligand displacement studies. Excellent for receptor subtype research.
Dr. Élise Rousseau • 2025-12-03Verified Purchase
Fast delivery to France, exceptional purity. Our sleep architecture studies with Orexin-B are highly reproducible.
Bonn Neurophysiology Institute • 2026-01-15Verified Purchase
Consistent quality for our hypothalamic neuron electrophysiology studies. Orexin-B-mediated depolarisation is clearly observed.
Dr. Sara Colombo • 2026-01-19Verified Purchase
Verified linear structure and correct sequence. Functional activity in our TMN neuron cultures is robust.
Turku Biomedical Sciences • 2025-12-10Verified Purchase
Orexin-B wake-promoting effects via OX2R are clearly separable from OX1R-mediated effects using this high-quality peptide.
Dr. Michal Dvořák • 2026-02-12Verified Purchase
Received in 4 days. Product quality excellent for our narcolepsy model research.
Linz Neuropharmacology Lab • 2026-03-15Verified Purchase
Orexin-B performs well in our dose-response studies. EC₅₀ values are consistent with published literature.
Dr. Bram Huijsman • 2025-11-24Verified Purchase
Excellent for OX2R pharmacological characterisation. Quality and analytical documentation are first-rate.
Granada Neuroscience Centre • 2026-02-28Verified Purchase
Multiple orders, consistently excellent. Our comparative orexin receptor studies produce reliable results.
Dr. Marta Kovalenko • 2025-10-09Verified Purchase
Easy ordering, quick delivery. Orexin-B quality confirmed by our independent mass spectrometry analysis.
Bern Neuroscience Institute • 2026-03-12Verified Purchase
High-quality Hypocretin-2 with comprehensive COA documentation. Highly recommended for sleep and arousal researchers.
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